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Design and Development of Biosensor System for Detection of Pesticides.

Pundareeka, B. L. (2012) Design and Development of Biosensor System for Detection of Pesticides. [Student Project Report]

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The objective is the development of an Immunobiosensor. Antibodies are produced against ethyl and methyl parathion, 2-4D and Atrazine. When pesticides enter the body. conditions for immobilization of the antibodies on suitable solid support have been standardized. The concept of detection of pesticides based on chemiluminescence (CL) where in the required sensitivity at sub-nanogram level could be detected is employed. A critical factor in lummuno sensors development is the stabilization of the enzyme conjugate at nanomole concentration for use with biosensor. This posed a considerable challenge ,But after several attempts this could also be achieved. Basic information on the production and immobilization of antibodies and date on several parametric optimization of the bioreactor column and sensor performance have been generated. It was felt that the results achieved so far should be pursued towards construction of an integrated biosensor device for application and commercialization . The technique involved in this project is effective and it is possible to produce the biosensor device for measurement of pesticide with adequate (ppt level ) sensitivity using the chemiluminescence method of detection.

Item Type: Student Project Report
Uncontrolled Keywords: Immunobiosensor, detection of pesticides
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 09 May 2012 08:57
Last Modified: 09 May 2012 08:57
URI: http://ir.cftri.com/id/eprint/10690

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