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Supercritical CO2 extraction of Mortierella alpina single cell oil: Comparison with organic solvent extraction.

Nisha, A. and Udaya Sankar, K. and Venkateswaran, G. (2012) Supercritical CO2 extraction of Mortierella alpina single cell oil: Comparison with organic solvent extraction. Food Chemistry, 133 . pp. 220-226.

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Abstract

Analysis of supercritical fluid extracted (SFE) and organic solvent extracted lipids from freeze dried Mortierella alpina biomass was carried out. The effect of biomass pre-treatment on effectual lipid recovery was also investigated. Results indicated that the methods employed viz., SFE and organic solvent (Sox Tec™) exhibited significant variation in lipid extraction efficacy. Efficacy of SFE on lipid recovery (38.73%) was significantly lower when compared to that of Sox Tec™ method (51.47%) because the organic solvent can also extract polar lipids. Biomass pretreatment by enzymatic method resulted in a substantial increase in lipid recovery. Maximum disruption of cell wall evidenced by scanning electron microscopy of treated cells warranted their higher mass transfer and efficient extraction. Characterisation of the extracted lipids by GC and GC–MS revealed considerable variation in the fatty acid profile. The FTIR spectrum indicated a prominent cis-stretching vibration, no evident trans- stretching vibration and no major discrepancies between the SFE and Sox Tec™ extracts.

Item Type: Article
Uncontrolled Keywords: Mortierella, Supercritical extraction, Sox Tec™ extraction Total lipids
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
600 Technology > 08 Food technology > 01 Analysis
Divisions: Food Engineering
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Feb 2012 05:51
Last Modified: 28 Dec 2016 13:00
URI: http://ir.cftri.com/id/eprint/10655

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