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Aflatoxin Determination in Black Tea (Camellia Sinensis) – Status and Development of a Protocol.

Prema, Viswanath and Divyashree, K. N. and Hemalatha, Govindegowda and Akshatha, M. Dattatreya (2012) Aflatoxin Determination in Black Tea (Camellia Sinensis) – Status and Development of a Protocol. Journal of Food Safety, 32. pp. 13-21.

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Abstract

Aflatoxins, toxic mold metabolites, are naturally contaminants of food and have been reported on tea (Camellia sinensis), but there are no validated methods published for its determination.Amethod has been standardized incorporating Tween- 20, a nonionic surfactant in the extraction solvent, with use of an immunoaffinity column for cleanup followed by postcolumnphotochemical derivatization reversedphase high-performance liquid chromatography.Acceptable recoveries of 76–100% were observed when spiked with aflatoxins B1, B2, G1 and G2. Analysis of market samples of black tea showed the presence of aflatoxigenic molds in 25.9% of samples, and aflatoxin B1 was detected in one sample at a level of 19.2 mg/kg. Aflatoxin production was observed in tea samples artificially inoculated with the conidia of an aflatoxigenic strain of Aspergillus flavus.Major portion of the aflatoxins added to the tea leaves remains in the residue when boiled with water, and 30.6% gets partitioned into the filtrate,which is the component used to prepare the beverage.

Item Type: Article
Uncontrolled Keywords: Tea (Camellia sinensis), fungal strains, aflatoxin
Subjects: 600 Technology > 07 Beverage Technology > 08 Tea
600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions: Food Safety Analytical Quality Control Lab
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Feb 2012 04:29
Last Modified: 19 Jun 2012 04:26
URI: http://ir.cftri.com/id/eprint/10650

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