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Production of esculin by hairy root cultures of chicory (Cichorium intybus L.cv. Lucknow local).

George, J. and Harsh Pal, Bais and Ravishankar, G. A. (1999) Production of esculin by hairy root cultures of chicory (Cichorium intybus L.cv. Lucknow local). Indian Journal of Experimental Biology, 37. pp. 269-273. ISSN 0019-5189

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Abstract

Hairy root cultures established with Agrobacterium rhizogenes LMG 150 of an Indian cultivar of chicory produced esculin, a dihydroxycoumarin monoglucoside used as skin protectant and a marker in microbiological media. Maximum biomass of 6.2±0.425 g/culture of transformed roots was obtained in full strength MS medium with 3% sucrose on 28th day. Esculin production was linked to growth and 100 g FW of transformed roots gave a maximum of 13.93 mg, corresponding to a ~13-fold increase as compared to that in 30-day-old non-transformed roots (0.72 mg), during the same period.

Item Type: Article
Uncontrolled Keywords: chicory, Cichorium intybus, Hairy root cultures, Esculin production
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Feb 2012 05:47
Last Modified: 28 Sep 2018 10:23
URI: http://ir.cftri.com/id/eprint/10635

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