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Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L.

Vinod, Kumar and Gururaj, H. B. and Narasimha Prasad, B. C. and Giridhar, P. and Ravishankar, G. A. (2005) Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L. Scientia Horticulturae, 106. pp. 237-246.

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Abstract

In vitro direct multiple shoot formation from seedling explants of Indian high pungent varieties of Capsicum annuum cv. Arka Abhir (AA) and Arka Lohit (AL) was successfully obtained. We were able to induce regeneration potency in these varieties by inverting the explant. Aseptically grown seedling explants with decapitated roots, apical meristem and cotyledonary leaves were inoculated in an inverted position in bud induction medium comprising of Murashige and Skoog’s basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid (MES) buffer along with 26.63 mM benzyl adenine (BA), 2.28 mM indole-3-acetic acid (IAA) and 10 mM silver nitrate. Profuse shoot bud induction with 20–25 shoot buds per explant was obtained. Supplementation of phloroglucinol in the bud induction medium resulted in 17 and 18% enhancement in bud induction response in Arka Abhir and Arka Lohit variety, respectively on the inverted hypocotyls. Auxin transport inhibitor triiodo benzoic acid (TIBA) in the bud induction medium resulted in induction of buds in a shorter period of 40–45 days when compared to bud induction (BI) medium which takes 55–65 days for bud induction. These buds were transferred to MS medium containing 2.8 mM gibberellic acid and 10 mM silver nitrate resulting in elongation of shoot buds. Transfer of shoots to MS basal medium induced rooting to produce plantlets. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. annuum tissues.

Item Type: Article
Uncontrolled Keywords: Adventitious shoot buds; Capsicum annuum; Direct organogenesis; Hypocotyl explants; Polarity
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Feb 2012 05:11
Last Modified: 28 Aug 2018 05:59
URI: http://ir.cftri.com/id/eprint/10634

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