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Indoleamines and calcium channels influence morphogenesis in in vitro cultures of Mimosa pudica L.

Ramakrishna, A. and Giridhar, P. and Ravishankar, G. A. (2009) Indoleamines and calcium channels influence morphogenesis in in vitro cultures of Mimosa pudica L. Plant Signaling and Behavior, 4 (12). pp. 1136-1141.

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Abstract

The present article reports the interplay of indoleamine neurohormones viz. serotonin, melatonin and calcium channels on shoot organogenesis in Mimosa pudica L. In vitro grown nodal segments were cultured on MS medium with B5 vitamins containing Serotonin (SER) and Melatonin (MEL) at 100 μM and indoleamine inhibitors viz. serotonin to melatonin conversion inhibitor p-chlorophenylalanine (p-CPA) at 40 μM, serotonin reuptake inhibitor (Prozac) 20 μM. In another set of experiment, calcium at 5 mM, calcium ionophore (A23187) 100 μM, and calcium channel blocker varapamil hydrochloride (1 mM) a calcium chelator EGTA (100 μM) were administered to the culture medium. The percentage of shoot multiplication, endogenous MEL and SER were monitored during shoot organogenesis. At 100 μM SER and MEL treatment 60% and 70% explants responded for shoot multiplication respectively. Medium supplemented with either SER or MEL along with calcium (5 mM) 75%–80% explants responded for organogenesis. SER or MEL along with calcium ionophore (A23187) at 100 μM 70% explants responded for shoot multiplication. p-CPA, prozac, verapamil and EGTA, shoot multiplication was reduced and endogenous pools of SER, MEL decreased by 40–70%. The results clearly demonstrated that indoleamines and calcium channels positively influenced shoot organogenesis in M. pudica L.

Item Type: Article
Uncontrolled Keywords: melatonin, serotonin, calcium channels, Mimosa pudica L, shoot multiplication, root induction
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Feb 2012 04:57
Last Modified: 15 Feb 2012 04:57
URI: http://ir.cftri.com/id/eprint/10632

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