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Calcium and calcium ionophore A23187 induce high-frequency somatic embryogenesis in cultured tissues of Coffea canephora P ex Fr.

Ramakrishna, Akula and Giridhar, P. and Ravishankar, G. A. (2011) Calcium and calcium ionophore A23187 induce high-frequency somatic embryogenesis in cultured tissues of Coffea canephora P ex Fr. In Vitro Cellular and Developmental Biology - Plant, 47. pp. 667-673.

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Abstract

High-frequency somatic embryogenesis was achieved in Coffea canephora using calcium ionophore A23187, which influences the influx of calcium into a cell. With 100 μM calcium ionophore and 5 mM calcium, 85% and 70% of cultures produced embryogenic tissue, with 105±7 and 95±8 primary embryos from each callus mass respectively. Medium supplemented with 100 μM EGTA (calcium chelator) or 1 mM verapamil (calcium channel blocker) significantly reduced somatic embryogenesis. Calcium imaging studies were done to determine the relationship between morphogenetic response and the cellular calcium levels. The calcium ionophore/calcium treatment was very effective in driving cellular machinery toward embryogenesis. The embryos were regenerated into plantlets when cultured onMS medium supplemented with 5 mM calcium/100 μM calcium ionophore A23187. Somatic embryogenesis-derived plants were successfully transferred to soil and grown to maturity in the field.

Item Type: Article
Uncontrolled Keywords: Coffea canephora Calcium ionophore (A23187) Calcium Calcium imaging Primary somatic embryogenesis Secondary somatic embryogenesis
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Feb 2012 04:11
Last Modified: 15 Feb 2012 04:11
URI: http://ir.cftri.com/id/eprint/10626

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