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Elicitation of thiophene production by cultured hairy roots of Tagetes patula.

Rajasekaran, T. and Madhusudan, R. and Ravishankar, G. A. (1999) Elicitation of thiophene production by cultured hairy roots of Tagetes patula. Acta Physiologiae Plantarum, 21 (3). 243-247 .

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Abstract

Hairy roots of Tagetes patula were initiated by infecting the seedlings with Agrobacterium rhizogenes. The hairy roots were grown in liquid medium MS ( Murashige and Skoog 1962) supplemented with sucrose. The roots were treated with three different elicitors obtained from mycelial culture of Aspergillus niger, Rhizopus oligosporus and Pencillium notatum. Accumulation of biomass and the production of thiophenes were studied over a period of six weeks in culture. The HPLC separation profile of the thiophenes indicated the presence of several structurally different thiophenes, ct-terthienyl being predominent. Maximum production of thiophene was recorded at the end of the fourth week in culture with a content of 0.138 % (w/w on dry weight basis). Treatment of hairy roots with mycelial extract of Aspergillus niger (1.5 % v/v) elicited an increase in thiophene content by 1.6 folds over the control.

Item Type: Article
Uncontrolled Keywords: Fungal elicitors, Root culture, Thiophene, Tagetes patula
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 13 Feb 2012 09:50
Last Modified: 21 Dec 2016 12:57
URI: http://ir.cftri.com/id/eprint/10619

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