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Expression of carotenoid biosynthetic pathway genes and changes in carotenoids during ripening in tomato (Lycopersicon esculentum).

Namitha, K. K. and Archana, Surya Narayana and Negi, P. S. (2011) Expression of carotenoid biosynthetic pathway genes and changes in carotenoids during ripening in tomato (Lycopersicon esculentum). Food and Function, 2. pp. 168-173.

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Abstract

To study the expression pattern of carotenoid biosynthetic pathway genes, changes in their expression at different stages of maturity in tomato fruit (cv. Arka Ahuti) were investigated. The genes regulating carotenoid production were quantified by a dot blot method using a DIG (dioxigenin) labelling and detection kit. The results revealed that there was an increase in the levels of upstream genes of the carotenoid biosynthetic pathway such as 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), 4-hydroxy-3-methyl-but-2-enyl diphosphate reductase (Lyt B), phytoene synthase (PSY), phytoene desaturase (PDS) and z-carotene desaturase (ZDS) by 2–4 fold at the breaker stage as compared to leaf. The lycopene and b-carotene content was analyzed by HPLC at different stages of maturity. The lycopene (15.33 � 0.24 mg per 100 g) and b-carotene (10.37 � 0.46 mg per 100 g) content were found to be highest at 5 days post-breaker and 10 days post-breaker stage, respectively. The lycopene accumulation pattern also coincided with the color values at different stages of maturity. These studies may provide insight into devising gene-based strategies for enhancing carotenoid accumulation in tomato fruits.

Item Type: Article
Uncontrolled Keywords: tomato fruit, carotenoid production, Lycopersicon esculentum
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
600 Technology > 08 Food technology > 23 Vegetables > 03 Tomato
Divisions: Human Resource Development
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 10 Feb 2012 06:12
Last Modified: 07 May 2012 05:38
URI: http://ir.cftri.com/id/eprint/10599

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