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Inhibition of Helicobacter pylori growth and its cytotoxicity by 2-hydroxy 4-methoxy benzaldehyde of Decalepis hamiltonii (Wight & Arn); a new functional attribute.

Srikanta, B. M. and Harish Nayaka, M. A. and Shylaja, M. Dharmesh (2011) Inhibition of Helicobacter pylori growth and its cytotoxicity by 2-hydroxy 4-methoxy benzaldehyde of Decalepis hamiltonii (Wight & Arn); a new functional attribute. Biochimie, 93. pp. 678-688.

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Abstract

Helicobacter pylori mediated gastric ulcer and cancers are common global problems since it was found to colonize in w50% of gastric ulcer/cancer patients. Decalepis hamiltonii, (Asclepiadaceae family) extracts have been depicted with medicinal properties supporting the traditional knowledge of health beneficial attributes of D. hamiltonii. Previously we have shown that both aqueous as well as methanol extracts of D. hamiltonii containing abundant phenolics with predominant levels (20e40% of total phenolics) of 2- hydroxy-4-methoxy benzaldehyde (HMBA). Despite higher levels, HMBA contributed very little to the antioxidant activity (<10%) when compared to other phenolic compounds in the extract. In the current study we attempted to explore antimicrobial property, particularly anti-H. pylori activity, since traditional users document D. hamiltonii as a fighter of microbial infections. HMBA was isolated from the roots of D. hamiltonii by hydrodistillation and cold crystallization method; identified by HPLC and characterized using ESI-MS and confirmed byNMR studies as a compound of molecular mass 152 Da. IsolatedHMBAwas found to inhibit the growth of H. pylori, a potential ulcerogen in a dose dependent manner with MIC of w39 mg/ mL as apposed to that of amoxicillin (MIC e 26 mg/mL) for which H. pylori is susceptible. Results were further substantiated by the lysis of H. pylori by electron microscopy and electrophoretic studies. Studies on the mechanism of action indicated the counteracting effect of vacuolating toxin (VacA) of H. pylori which otherwise would lead to host cell cytotoxicity. Further the increased binding ability of HMBA to DNA and protein offered an impact on DNA protectivity and bioavailability. Results for the first time provide a direct evidence for anti-microbial attribute of HMBA. Insignificant antioxidant attribute of HMBA also reveals the anti-H. pylori activity via mechanisms other than antioxidative routes.

Item Type: Article
Uncontrolled Keywords: 2-Hydroxy-4-methoxy benzaldehyde, Helicobacter pylori Decalepis hamiltonii, Cytoprotection, Gastric ulcer
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs
600 Technology > 01 Medical sciences > 15 Pharmacology
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 10 Feb 2012 04:17
Last Modified: 22 Oct 2018 05:50
URI: http://ir.cftri.com/id/eprint/10593

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