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Studies on effect of additives on protein profile, microstructure and quality characteristics of pasta.

Purnima, C. and Ramasarma, P. R. and Prabhasankar, P. (2012) Studies on effect of additives on protein profile, microstructure and quality characteristics of pasta. Journal of Food Science and Technology, 49 (1). pp. 50-57. ISSN 0022-1155

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Abstract

Wheat storage proteins play a vital role in pasta making quality. In the present study, SDS-PAGE, Gel filtration chromatography and Scanning electron microscopy techniques were employed to understand the changes in the wheat protein fractions and their interactions with additives namely Sodium Steroyl Lactate (SSL), Glycerol Monostearate (GMS) and Hydroxy Propyl Methyl Cellulose (HPMC) during processing of pasta. SDS-PAGE studies indicated changes inHighMolecularWeight Glutenin (HMW) fractions during drying stages of pasta preparation and in cooked pasta samples. In uncooked pasta, gel filtration patterns showed four peaks corresponding to different storage proteins whereas in the case of cooked pasta, these peaks were merged into three peaks. Pasta quality characteristics studies indicated that pasta with HPMC was found to have minimum percentage of cooking loss (5.6%), increased cooked weight (82 g), firmness (2.97 N) and high overall quality score (27) than GMS, SSL and control. Microstructure studies confirm the beneficial effect of HPMC. The present study indicated that HPMC is better additive for pasta manufacture followed by GMS. This could be due to interaction of HPMC with starch and protein matrix is different from that of GMS and SSL.

Item Type: Article
Uncontrolled Keywords: Emulsifiers Wheat proteins Scanning electron microscopy Electrophoresis Gel filtration
Subjects: 600 Technology > 08 Food technology > 26 Bakery products
600 Technology > 08 Food technology > 31 Food Additives
Divisions: Flour Milling Bakery and Confectionary Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 Jan 2012 04:19
Last Modified: 30 Dec 2016 11:01
URI: http://ir.cftri.com/id/eprint/10585

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