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Exploration of ricebran, an agro-industry residue, for the production of intra- and extra-cellular polymers by Sinorhizobium meliloti MTCC 100.

Saranya Devi, E. and Vijayendra, S. V. N. and Shamala, T. R. (2012) Exploration of ricebran, an agro-industry residue, for the production of intra- and extra-cellular polymers by Sinorhizobium meliloti MTCC 100. Biocatalysis and Agricultural Biotechnology, 1 (1). pp. 80-84.

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Abstract

The present work was focused on simultaneous production of certain intra- and extra-cellular polymers by fast growing Sinorhizobium meliloti MTCC 100 using ricebran, a low cost agro-industry residue, in hydrolyzed form to enhance product yields. The culture produced 3.63 g/L of biomass, 1.75 g/L of intra-cellular polymer (polyhydroxyalkanoate, PHA) and 1.2 g/L of extra-cellular polymer (exopolysaccharide, EPS) in control, polymer production (PP) medium. Supplementation of 20% ricebran hydrolysate (RBH) to PP medium at 0 h resulted in increased production of biomass, PHA and EPS (5.92, 2.71 and 2.01 g/L, respectively). Addition of RBH at after 24 h of fermentation increased the amount of EPS by 5 folds after 72 h at 30 °C. An initial pH of 7.0 and fermentation temperature of 30 °C were found to be optimum for the production of biomass as well as both the biopolymers. The biomass, PHA and EPS contents increased with the increase in fermentation period from 24 h to 72 h, with a maximum biomass of 7.45 g/L and PHA of 3.60 g/L. With further incubation to 96 h EPS production increased to 11.8 g/L. Gas chromatography and Fourier transform infra red spectroscopy of the PHA indicated it to be a copolymer of polyhydroxybutyrate and polyhydroxyvalerate.

Item Type: Article
Uncontrolled Keywords: Characterization; Exopolysaccharide; Polyhydroxyalkanoate; Ricebran hydrolysate; Sinorhizobium meliloti
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 27 Polymer Chemistry
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Jan 2012 09:27
Last Modified: 16 Jan 2017 12:44
URI: http://ir.cftri.com/id/eprint/10564

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