[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr.

Vinod, Kumar and Giridhar, P. and Chandrashekar, A. and Ravishankar, G. A. (2008) Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr. Acta Physiologiae Plantarum, 30. pp. 217-223.

[img] PDF
Acta Physiologiae Plantarum 2008 28 122.pdf - Published Version
Restricted to Registered users only

Download (383kB) | Request a copy

Abstract

The influence of polyamines, polyamine inhibitors and ethylene inhibitors were tested in Coffea canephora for in vitro morphogenetic response and caffeine biosynthesis. Coffea canephora produced nonembryogenic and embryogenic calli. Somatic embryos were produced only from the embryogenic callus. Endogenous polyamine pools were estimated in these tissues. Somatic embryos were subjected to secondary embryogenesis under the influence of putrescine, silver nitrate and specific inhibitors of polyamine biosynthesis. Estimation of endogenous total polyamines revealed that embryogenic callus contained 11-fold more spermine and 3.3-fold higher spermidine when compared to non-embryogenic callus. Incorporation of polyamines resulted in 58% explant response for embryogenesis when compared to control with 42% response. Incorporation of silver nitrate resulted in 65% response for embryogenesis. Incorporation of polyamine biosynthetic pathway inhibitors DFMO and DFMA resulted in 83% reduction in embryogenic response with concomitant increase in caffeine levels by two-fold as compared to control. These results have clearly demonstrated that polyamines play a crucial role in embryogenesis and caffeine biosynthesis.

Item Type: Article
Uncontrolled Keywords: Caffeine � Coffea canephora � Polyamines � S-adenosyl-L-methionine � Somatic embryogenesis
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plant Cell Biotechnology
Depositing User: Users 7 not found.
Date Deposited: 12 Jan 2012 09:42
Last Modified: 22 Oct 2018 09:43
URI: http://ir.cftri.com/id/eprint/10488

Actions (login required)

View Item View Item