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Transformation of L-Tyrosine to L-Dopa by a Novel Fungus, Acremonium rutilum , Under Submerged Fermentation.

Krishnaveni, R. and Vandana, Rathod and Thakur, M. S. and Neelgund, Y. F. (2009) Transformation of L-Tyrosine to L-Dopa by a Novel Fungus, Acremonium rutilum , Under Submerged Fermentation. Current Microbiology , 58 (2). pp. 122-128.

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Abstract

The present study deals with the transformation of L-tyrosine to L-dopa by Acremonium rutilum, a fungal tyrosinase producer, isolated from decomposed banana stud. This appears to be the first report on A. rutilum as a polyphenoloxidase producer with both cresolase and catecholase activity. Enriched Czapek-Dox agar was used for plate assay screening. Enriched potato dextrose broth was used for optimization studies, which induced high levels of L-dopa under submerged fermentation. A. rutilum gave the maximum L-dopa production (0.89 mg/ml) and tyrosinase activity (1095 U/mg) under the optimized parameters, that is, a temperature of 25°C, pH 5.5, an inoculum size of 2.5 ml, and an incubation time of 72–120 h, with L-tyrosine (5 mg/ml) as substrate. Five resolved bands, with Rf values of 0.73, 0.60, 0.54, 0.37, and 0.26, were observed, which confirmed the presence of L-dopa. This study involves the elevated profile of L-dopa production. Such study is needed, as L-dopa has the ability to control Parkinson’s disease.

Item Type: Article
Uncontrolled Keywords: Acremonium rutilum; Parkinson’s disease; fungal tyrosinase producer
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 09 Aug 2011 07:16
Last Modified: 28 Dec 2011 10:28
URI: http://ir.cftri.com/id/eprint/10438

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