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Rapid screening of enzyme inhibitors using PRofiling of Enzyme-Metabolite Assay by HPLC (PREMA-HPLC).

Vasantha, K. Y. and Murugesh, C. S. and Sattur, A. P. (2012) Rapid screening of enzyme inhibitors using PRofiling of Enzyme-Metabolite Assay by HPLC (PREMA-HPLC). Journal of Enzyme Inhibition and Medicinal Chemistry, 27 (1). pp. 132-137.

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Abstract

A number of isolates from different ecosystems were screened for their ability to inhibit tyrosinase resulting in the selection of isolate CFR 101, which showed an inhibition of 72%. The metabolites present in the crude extract of the selected isolate was profiled through high-performance liquid chromatography (HPLC) before the enzyme inhibition assay to reveal a 66% decrease in area of the peak at room temperature for 13.9 min, after the assay. Upon purification, this peak was identified as kojic acid, a known inhibitor of tyrosinase. This unique technique of combining a reaction assay mixture with HPLC profile wherein inhibitors can be rapidly pinpointed in crude extracts addresses the drawback of rapid chemical high-throughput screening (HTS) systems, which is limited to the chemical nature of metabolites without any evidence of their biological activities.

Item Type: Article
Uncontrolled Keywords: Tyrosinase inhibitors, HPLC, screening, kojic acid
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography
600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Aug 2011 07:20
Last Modified: 24 Jan 2012 06:50
URI: http://ir.cftri.com/id/eprint/10424

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