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Optimization of Media Components for Enhanced Arachidonic Acid Production by Mortierella alpina under Submerged Cultivation.

Nisha, Alpettiyil and Rastogi, N. K. and Venkateswaran, G. (2011) Optimization of Media Components for Enhanced Arachidonic Acid Production by Mortierella alpina under Submerged Cultivation. Biotechnology and Bioprocess Engineering, 16. pp. 229-237.

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Abstract

Mortierella alpina, an oleaginous zygomycete is a potent producer of arachidonic acid, the pharmaceutically and nutraceutically important polyunsaturated fatty acid of the n-6 series. It serves a wide variety of purposes, from being a purely structural element in phospholipids to being involved in signal transduction, and as a substrate for a host of derivatives involved in second messenger function. Arachidonic acid has applications in diverse areas including infant and geriatric nutrition. In the present study, the interactive effects of four major media constituents on arachidonic acid production were investigated by applying a central composite rotatable design (CCRD) and response surface methodology (RSM). The independent variables, which were selected byconcentrations of glucose, corn solids, KH2PO4, and KNO3 influenced the production of biomass, total lipid, and arachidonic acid by M. alpina. A second-order polynomial was fitted by multiple regression analysis of the experimental data. The optimum conditions (glucose 10.0 g/L, corn solids 5.0 g/L, KH2PO4 1.0 g/L, and KNO3 1.0 g/L) resulted in maximum production of arachidonic acid (1.39 g/L) and the corresponding biomass and total lipid concentrations were 12.49 and 5.87 g/L, respectively.

Item Type: Article
Uncontrolled Keywords: arachidonic acid, media optimization, Mortierella alpina, response surface methodology
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions: Food Engineering
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 05 Aug 2011 10:56
Last Modified: 30 Apr 2012 06:27
URI: http://ir.cftri.com/id/eprint/10412

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