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Extraction, purification and concentration of partially saturated canthaxanthin from Aspergillus carbonarius.

Krupa, D. and Nakkeeran, E. and Kumaresan, N. and Vijayalakshmi, G. and Subramanian, R. (2010) Extraction, purification and concentration of partially saturated canthaxanthin from Aspergillus carbonarius. Bioresource Technology, 101 (19). 7598-7604 .

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Abstract

A mutant Aspergillus carbonarius produces partially saturated canthaxanthin (PSC; C40H62O2) during submerged fermentation. The pigment was extracted from dried biomass using various organic solvents and purified using nanofiltration (NF) and nonporous membranes. Particle size had a great influence; PSC extractability from fines fraction of biomass (75–105 μm) was 1.5-fold higher compared to the coarse fraction (850–920 μm) in ethanol. Among the four solvents, hexane exhibited the highest PSC extractability of 5.83 mg/g and purity of 32 mg/g. On a relative scale, the extraction performance of hexane, acetone, methanol and ethanol were in the order 100, 16.1, 7.5 and 5.4. An assessment based on enrichment factor and permeate flux revealed notable performance with NF-250 membrane in ethanol extract followed by NF-200 and NF-GKSS membranes in methanol extract. These results suggested the suitability of hexane for extraction followed by alcohol phase purification and concentration employing NF.

Item Type: Article
Uncontrolled Keywords: Aspergillus carbonarius; Partially saturated canthaxanthin; Extraction; Nanofiltration; Purification
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Aug 2011 09:54
Last Modified: 03 Aug 2011 09:54
URI: http://ir.cftri.com/id/eprint/10382

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