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Influence of different ethylene inhibitors on somatic embryogenesis and secondary embryogenesis from Coffea canephora P ex Fr.

Vinod, Kumar and Ramakrishna, A. and Ravishankar, G. A. (2007) Influence of different ethylene inhibitors on somatic embryogenesis and secondary embryogenesis from Coffea canephora P ex Fr. In Vitro Cellular and Developmental Biology - Plant, 43. pp. 602-607.

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Abstract

The effect of cobalt chloride, salicylic acid, and silver nitrate for embryogenesis was studied in in vitro cultures of Coffea canephora. Murashige and Skoog (in Physiol. Plant. 15:473–497, 1962) medium containing 20 and 40 μM either of cobalt chloride, silver nitrate, or salicylic acid supplemented with 1.1 μM N6 benzyladenine and 2.85 μM indole-3-acetic acid was used for the study. At 20 and 40 μM silver nitrate treatment, 35–48% explants responded for embryogenesis, and 38±7 and 153±27 embryos were produced from each callus mass, respectively, whereas only 5% control explants responded on medium devoid of silver nitrate, cobalt chloride, or salicylic acid. Secondary embryogenesis was observed in 70–90% of the explants, and around 100–150 embryos were produced from each explant cultured on a medium containing silver nitrate, and only a 3% response was noticed in control embryo explants. Yellow friable embryogenic calluses were obtained from the cut edges of most of the tissues grown in a medium supplemented with cobalt chloride. The results clearly demonstrated that, among the tested ethylene inhibitors, silver nitrate is very effective in reprogramming the cellular machinery toward embryogenesis.

Item Type: Article
Uncontrolled Keywords: Coffea canephora . Ethylene inhibitors . Somatic embryogenesis . Secondary embryogenesis . Coffee . Tissue culture
Subjects: 600 Technology > 07 Beverage Technology > 04 Coffee
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 29 Jul 2011 11:24
Last Modified: 30 Dec 2016 08:53
URI: http://ir.cftri.com/id/eprint/10323

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