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Genetic analyses of micropropagated and regenerated plantlets of banana as assessed by RAPD and ISSR markers.

Venkatachalam, L. and Sreedhar, R. V. and Bhagyalakshmi, N. (2007) Genetic analyses of micropropagated and regenerated plantlets of banana as assessed by RAPD and ISSR markers. In Vitro Cellular and Developmental Biology - Plant, 43. pp. 267-274.

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Abstract

A cultivar of dessert banana, namely, Nanjanagudu Rasabale (NR), classified under group “silk” (of genotype AAB), is seriously under the threat of extinction due to its susceptibility to bacterial wilt and bunchy-top virus disease. A regeneration protocol using tissue culture method was developed (Venkatachalam et al. 2006), where a large number of plantlets were regenerated from leaf base explants. Simultaneously, a micropropagation protocol was also developed where high levels of up to 53.28 μM of benzylamino purine (BAP) and 55.80 μM of kinetin (Kn) were used. The progressive increase of cytokinins levels resulted in concomitant increase in shoot number, with a maximum of 80 shoot buds per segment in BAP (31.08 μM). The plantlets were analyzed for their genetic stability using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. A total of 50 RAPD and 12 ISSR primers resulted in 625 distinct and reproducible bands showing homogeneous RAPD and ISSR patterns. Band intensity histogram of each gel confirmed their monomorphic nature with no genetic variation among the plantlets analyzed. The present study has established for the first time that the regeneration and rapid micropropagation protocol developed through the present study will be of great use in conserving the endangered cultivar – NR – without risk of genetic instability.

Item Type: Article
Uncontrolled Keywords: Banana . Endangered . Genetic stability . ISSR . Micropropagation . RAPD . Regeneration
Subjects: 600 Technology > 08 Food technology > 24 Fruits > 02 Banana
500 Natural Sciences and Mathematics > 07 Life Sciences > 02 Evolution & genetics
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 29 Jul 2011 11:15
Last Modified: 28 Dec 2011 10:26
URI: http://ir.cftri.com/id/eprint/10321

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