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Characterization of a new acidic heteropolysaccharide produced by a native isolate of Lactobacillus sp. CFR-2180

Mohan, R. and Vijayendra, S. V. N. (2010) Characterization of a new acidic heteropolysaccharide produced by a native isolate of Lactobacillus sp. CFR-2180. In: Handbook of Carbohydrate Polymers: Development, properties and applications. Nova Science Publishers, USA, pp. 757-766. ISBN 978-1-60876-367-2

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Polysaccharides are being used in food and allied industries as texture improving agents, stabilizers, for the preparation of the edible films etc. Plant and seaweed derived polysaccharides suffer lack of assured supply and variations in quality. Microbial exopolysaccharides (EPS) provide a valid alternative. An EPS producing lactic acid bacterium was isolated from cabbage and identified as Lactobacillus sp. CFR-2180. Production of 22 g/L of EPS in 24 h at 30°C was noticed. The EPS had 65% total carbohydrates, 0.7% protein, 10% uronic acid and 2.0% moisture. Analysis by gas chromatography revealed that the EPS is a heteropolysaccharide with the presence of mannose, galactose and glucose in a ratio of 1:7:5, respectively. Gel permeation chromatography and HPLC analysis of the EPS indicated presence of multiple peaks with molecular weight ranging from 1.8 x 104 to 2.5 x 106 Da., confirming the heterogeneity of the EPS. The results of the preliminary characterization of the EPS indicated that it is a new EPS that has not been reported earlier.

Item Type: Book Section
Uncontrolled Keywords: exopolysaccharide, fermentation, characterization, identification, heteropolysaccharides, uronic acid
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Jul 2011 09:47
Last Modified: 28 Dec 2016 08:43
URI: http://ir.cftri.com/id/eprint/10309

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