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Stress induced biochemical and molecular changes in Coffea canephora.

Beulah, K.C. (2011) Stress induced biochemical and molecular changes in Coffea canephora. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Stress is an important factor that dictates various changes at physiological level in plants. The stress may be of physical or environmental or biotic or abiotic type. Both biotic and abiotic stress play a crucial role in plants as they not only affect the growth index of the plant but are also able to trigger the production of signal molecules for defense against invading microbes or produce secondary metabolites which may be specific or non- specific. Coffee is prone to various biotic or abiotic stress factors, due to this there will be significant variation in bioactive potential of coffee beans. In order to know this at both biochemical and molecular level a study has been taken up to track the caffeine alkaloids profile under salicylic acid and methyl jasmonate administration which are important regulators of defense and wounding response in plants. In this study we found that under both conditions the levels of caffeine increased correlating with increased biosynthetic activity as shown by the up-regulation of the biosynthetic genes of the core caffeine biosynthetic pathway. These genes which code for methylxanthine synthase, theobromine synthase and caffeine synthase may be directly under the regulation of MeJ and SA or the signaling moieties therein. MeJ had a more profound effect on accumulation of caffeine but for a shorter duration. These results suggest that caffeine may play an important role in defense response of coffee thus strengthening the defense hypothesis. The exact role of these alkaloids in plant defense response however remains to be illustrated. Another important finding was the accumulation of theobromine in salt stress although the caffeine levels reduced in prolonged exposure to salinity stress. Molecular data however is not available at present for salinity related reduction of caffeine biosynthesis.
Uncontrolled Keywords: Coffee, biotic, abiotic stress, secondary metabolites
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Jul 2011 05:18
Last Modified: 28 Dec 2011 10:26
URI: http://ir.cftri.com/id/eprint/10259

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