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Partial characterization of polyhydroxyalkanoates produced by Sinorhizobium meliloti MTCC 100 by using different agro residues.

Sushma, M. (2011) Partial characterization of polyhydroxyalkanoates produced by Sinorhizobium meliloti MTCC 100 by using different agro residues. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: The use of synthetic polymer has become a major problem in our daily life. These plastics not only take many decades to be decomposed in nature, but they are responsible for a great contribution in the environment pollution by producing toxins during the process of degradation. Polyhydroxyalkanoates are biodegradable and biocompatible thermoplastics which degrade easily by microorganism. They can be used in various ways similar to non biodegradable petrochemical sources. The main objective of the present work was to study about the partial characterization of the polyhydroxyalkanoates produced by Sinorhizobium meliloti MTCC100 using different agro-industry residues such as cotton seed oil cake extract, coconut oil cake extract, gingelly oil cake extract, groundnut oil cake extract, rice bran oil cake extract, sunflower oil cake extract as supplement. The work was initially started with supplementing the PHA production medium with different agro residues. About six different oil cake extracts such as CSOC, COC, GOC, GROC, RBOC and SOC were used for the production of PHA. Among six oil cake extracts cotton seed oil cake extract and rice bran oil cake extract were found to be the best. And the PHA produced by these oil cake extracts was subjected to direct chloroform extraction and partially characterized. The quality of polymers was characterized by Crotonic acid assay, UV-Spectrophotometry (for the determination of λ-max), FT-IR and Gas chromatography. By Crotonic acid assay, concentration of all the oil cake extracts was determined and it showed that gingelly oil cake extract and sunflower oil cake extract were pure when compared to other oil cake extracts. However, PHA obtained from production medium was more pure than gingelly oil cake extract and sunflower oil cake extract. In FT-IR analysis that the spectrum for CSOC, COC, GROC, RBOC and SOC indicates as PHB molecules. In Gingelly oil cake extract the bands at 2931 cm-1 indicates that it may be scl-co-mcl PHA. GC analysis showed that many of the polymers obtained were polyhydroxyvalerate. All the supplements showed retention time around 8th minute. None of the supplements showed retention time around 11th minute. But supplements GROC and RBOC showed a peak with retention time around 28th minute and it is due to mcl-PHA molecule. PHA films formed after sodium chloride extraction showed good quality film more flexibility. Hence, it can be concluded that the supplements CSOC and ROC were co-polymers of PHA and mcl-PHA molecules and supplements COC, GOC, GROC and SOC were PHB molecules. In CSOC the area ( % ) of PHA is 51.74 and in ROC the area (% )of PHA is 45.22. From this study it can be concluded that agro-industrial residues can be exploited for enhance production biopolymers and to reduce the production cost. PHAs are gaining serious consideration as substitute for non-biodegradable petrochemical plastics. All the experiments were carried out in shake flask level, production of biopolymers can be further optimized using fomenters. The recovery and purification of substrates like agro-industry residues can substantially reduce the production cost. Further studies may also required for complete characterization of the biopolymers produced by Sinorhizobium meliloti MTCC 100.
Uncontrolled Keywords: Polyhydroxyalkanoates, Sinorhizobium meliloti, synthetic polymer, agro-industry residues
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 27 Polymer Chemistry
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Jul 2011 05:09
Last Modified: 28 Dec 2011 10:26
URI: http://ir.cftri.com/id/eprint/10258

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