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Agro residues a s a carbon source for the production of polyhydroxyalkanoates by Sinorhizobium meliloti MTCC.

Nanditha Rao, D. (2011) Agro residues a s a carbon source for the production of polyhydroxyalkanoates by Sinorhizobium meliloti MTCC. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: The accumulation of petrochemical plastic waste in the environment is an increasing problem. These plastics have become an important commodity to enhance the comfort and quality of life. These plastics not only take many decades to be decomposed in nature but also produce toxins during the process of degradation. In order to solve this problem biopolymers like polyhydroxyalkanoates, which are similar to petrochemical sources, biodegradable non toxins, ecofriendly and can be easily degraded by microbes, are being explored The main objective of the present work was to produce PHA by the bacteria Sinorhizobium meliloti MTCC 100 using agro industrial residues as supplements and characterizing the polymer by GC. For the production of PHA, aqueous extracts of various oil cakes i.e., SOC, GOC were supplemented to the production medium i.e., SOC, GOC. The optimization of PHA includes effect of pH, sugar concentration, concentration of oil cake extract, time etc., The medium supplemented with carbon source i.e., sucrose, ammonium sulphate as nitrogen source which play an important role in cell growth of the bacteria. In the present experiment of PHA production, Sino rhizobium meliloti utilizes the oil cakes extract, in order to increase the elasticity of bioplastics by increasing the side chain of fatty acids with the help of lipid present in the oil cakes extract. Through the experimental analysis it can be concluded that the PHA obtained can be produced by supplementing different oil cakes extract which likely produce desired polymers. The bio polymer production rate was 1.72 w/w, 40.3% PHA yield in case of SOC which was more compared to that of GOC which produced 1.13 g/L PHA with 22.6% PHA yield. In an experiment to know the effect of different pH, it was found to be 1.4 g/L PHA, 47.1% PHA yield at 72 hours, which was less compared to the result of 48 hours at pH 6 PHA 1.2 g/L 30% PHA yield.
Uncontrolled Keywords: PHA, Sinorhizobium meliloti MTCC 100, polyhydroxyalkanoates
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 27 Polymer Chemistry
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jul 2011 09:48
Last Modified: 28 Dec 2011 10:25
URI: http://ir.cftri.com/id/eprint/10241

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