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Identification of Staphylococcus Species by using Species Specific Primers.

Sandhya Rani, S. (2011) Identification of Staphylococcus Species by using Species Specific Primers. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Staphylococcus species is an important group of food borne pathogen, but not all the staphylococci are pathogenic. Therefore correct identification of Staphylococcus species is a challenging task. Currently, convectional phenotypic characterization is used for the identification of staphylococcus species. Several biochemical based commercial kits and automated systems are also available for identification. Phenotypic characterizations have limitation due to variable expression of characters and ambiguity in the interpretation of end point reactions. The use of nucleic acids targets with their high sensitivity and specificity may provide an alternative means of accurately identifying Staphylococcus species. Therefore, in the present investigation an attempt was made to identify various Staphylococcus species using nucleic acid based targets. Species specific primers were used for identification of different species. DNA extracted from food isolates was used as a template and PCR was preformed using primers for all the species. Among different food isolates, isolate 202 was identified as S.gallinarum and isolate 314 as S.felis. Remaining isolates showed cross reactivity, which may be due to closeness among the isolates. Further work is needed to analyse the closeness among these species using other molecular tools.
Uncontrolled Keywords: Staphylococcus, food borne pathogen, nucleic acid based targets
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
600 Technology > 01 Medical sciences > 07 Food borne disease
Divisions: Human Resource Development
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jul 2011 09:38
Last Modified: 28 Dec 2011 10:25
URI: http://ir.cftri.com/id/eprint/10239

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