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Neuroprotective efficacy of ferulic acid against neurotoxicant rotenone in mice.

Rajpreet, Singh (2011) Neuroprotective efficacy of ferulic acid against neurotoxicant rotenone in mice. [Student Project Report]

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This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: OUTLINE: Ferulic acid (FA) is the hydroxycinnamic acid derivative which exhibits a wide range of therapeutic effects that are attributed to its potent antioxidant capacity. Exposure of agricultural pesticide like rotenone (ROT) induces oxidative stress and significantly inhibits complex I enzyme leading to mitochondrial dysfunction. OBJECTIVE: To assess the neuroprotective efficacy of FA against the ROT induced oxidative damage and mitochondrial dysfunction in mice. METHODOLOGY: FA was orally administered at doses (25 and 50 mg/kg b.w.) for short (1 week) and long term (4 weeks). Endogenous oxidative markers (ROS, MDA, HP, PC and NO) levels, activity of antioxidant enzymes (SOD, CAT, GST) were determined. Sub lethal doses of ROT (0.5 and 1 mg/kg i.p b.w) were administered to induce oxidative insult and mitochondrial dysfunction in various brain regions (Ct, Cb, Hp and St). Finally, oxidative marker levels and mitochondrial impairment (MTT, SDH, ETC enzymes) were estimated. Potential of FA (50 mg/kg b.w, 7 days) to ameliorate rotenone induced changes in redox status, mitochondrial function, cholinergic effect and monoamine levels was assessed. RESULTS: Oral supplementation of FA (at both doses) for short term (1 week) resulted in significant reduction of oxidative marker level and increase in the antioxidant enzyme activities. Data on oxidative marker and antioxidant enzyme levels with long term supplementation of FA was significantly enhanced concentration dependently. ROT administration resulted in significant oxidative damage, mitochondrial dysfunction and depleted dopamine levels (striatal region). Moreover, mice orally supplemented with FA with ROT showed significant protection with decrease oxidative marker levels, attenuation of altered levels of antioxidant enzymes and activities of ETC complex enzymes. INTERPRETATION AND CONCLUSION: Collectively, our findings summarizes that FA modulates the markers of oxidative stress in brain tissues and has ability to attenuate the ROT induced oxidative damage and mitochondrial impairments in both cytosol and mitochondrial fractions. Hence, it can hypothesized that the neuroprotective role of FA is due to its free radical scavenging property or alternatively to enhance the antioxidant defense.
Uncontrolled Keywords: Ferulic acid, rotenone, oxidative stress, mitochondrial dysfunctions, ETC enzymes (complex I-III, II-III)
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
600 Technology > 01 Medical sciences > 17 Toxicology
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jul 2011 05:03
Last Modified: 28 Dec 2011 10:25
URI: http://ir.cftri.com/id/eprint/10225

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