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Drought-Induced Alterations In Growth, Osmotic Potential and In Vitro Regeneration of Soybean Cultivars

Sakthivelu, G. and Akitha Devi, M. K. and Giridhar, P. and Rajasekaran, T. (2008) Drought-Induced Alterations In Growth, Osmotic Potential and In Vitro Regeneration of Soybean Cultivars. General and Applied Plant Physiology, 34 (1-2). p. 103.

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Abstract

Environmental stresses result in water deficiency for the plants, thus impairing its numerous biological roles. In vitro screening for stress tolerance will have its significance in identifying cultivars with optimal stress tolerance and productivity. In the present study, drought-induced alterations in growth, osmotic potential and in vitro shoot multiplication of soybean cultivars from India and Bulgaria were assessed with different concentrations of polyethylene glycol (PEG 6000 MW). In vitro callus cultures of both Indian (Hardee and JS 335) and soybean cultivars grown in Bulgarian (Collina and Korada) showed a reduction in callus growth during PEG treatment as compared with the control. The presence of PEG in the medium elevated dry matter content in all treatments compared with the control. Cotyledonary nodal explants subjected to 6 % PEG 6000 treatments resulted in 57.8 % and 68.5 % reduction in shoot induction in case of Hardee and JS 335, respectively, compared with the control. Our results can be used for in vitro screening and manipulations of soybean cultivars for improvement of drought tolerance.

Item Type: Article
Uncontrolled Keywords: drought, in vitro screening, polyethylene glycol, soybean.
Subjects: 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Jun 2011 11:24
Last Modified: 29 Aug 2018 06:48
URI: http://ir.cftri.com/id/eprint/10163

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