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Screening aflatoxigenic fungi in select spice samples by PCR.

Latha, R. and Dr., Shadanaika and Manonmani, H. K. and Chandrashekar, A. and Rati Rao, E. (2006) Screening aflatoxigenic fungi in select spice samples by PCR. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 8 (1). pp. 35-40.

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Abstract

Aflatoxins are potent secondary metabolites produced commonly by Aspergillus flavus, A parasiticus and A. nomius. Primers were designed specifically for O-methyl transferase (omt) gene that encodes the enzyme involved in the conversion of sterigmatocystin to O-methylsterigmatocystin of aflatoxin biosynthetic pathway. Experimental conditions were standardized for optimum PCR. The omt primer pair gave specific PCR amplification for aflatoxigenic Aspergilli. It did not give amplification for non-aflatoxigenic. A. flavus and other fungi such as A. oryzae, A glaucus, Fusarium, Penicillium and for Rhizopus spp. Market samples of spices were screened for contamination by aflatoxigenic fungi both by traditional methods and by PCR. They gave positive amplification with enrichment technique for aflatoxigenic fungi at spore count of greater than or equal to 10<sup>1</sup> cfu/g.

Item Type: Article
Uncontrolled Keywords: Aspergillus, Fusarium; Penicillium, Rhizopus, Spores
Subjects: 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 03 Fungi
600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions: Human Resource Development
Depositing User: Users 16 not found.
Date Deposited: 15 Jun 2011 11:15
Last Modified: 21 Dec 2016 12:23
URI: http://ir.cftri.com/id/eprint/10115

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