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Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation

Ashwani, Sharma and Vinod, Kumar and Giridhar, Parvatam and Ravishankar, G. A. (2008) Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electronic Journal of Biotechnology, 11 (2). pp. 1-6.

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Abstract

The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 μM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 μM) and cobalt chloride (30 μM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in transformation of capsicum using germ free in vitro flowers.

Item Type: Article
Uncontrolled Keywords: Capsicum frutescens, cobalt chloride, in vitro flowering, pollen transformation, regeneration, silver nitrate.
Subjects: 600 Technology > 08 Food technology > 30 Spices/Condiments
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jun 2011 06:40
Last Modified: 28 Dec 2011 10:23
URI: http://ir.cftri.com/id/eprint/10078

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