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Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L.

Thimmaraju, R. and Bhagyalakshmi, N. and Venkatachalam, L. and Sreedhar, R. V. (2006) Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electronic Journal of Biotechnology, 9 (5). pp. 513-521.

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Abstract

Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional *Corresponding author enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 μM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by glutathione (3.44-fold) and Rhizophus oligosporus (3.09-fold). Among abiotic elicitors, thidiazuron, Mg and Ca salts elicited 2.49, 3.03 and 2.8 fold activities respectively. While most of the biotic elicitors were effective when added on 15th day of culture, the abiotic elicitors were effective when added on 20th day. Combination of highly effective elicitors indicated that glutathione (1 mM) and dry cell powder of R. oligosporus caused a 4-fold enhancement in enzyme activity, accounting for 10.9 x 106 U L-1. The present study is the first report on red beet hairy roots where a large number of elicitors have been systematically screened and their probable involvements in eliciting POD activities have been discussed.

Item Type: Article
Uncontrolled Keywords: Aspergillus, calcium, culture filtrate, dried cell powder, elicitor, glutathione, methyljasmonate, Rhizophus, thidiazuron
Subjects: 600 Technology > 08 Food technology > 23 Vegetables > 06 Beet Root
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jun 2011 06:31
Last Modified: 06 Jul 2015 12:37
URI: http://ir.cftri.com/id/eprint/10076

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