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The resolution of bimodal peaks in the analytical ultracentrifuge in the presence anf absence of ligands.

Prakash, V. and Timasheff, S. N. (1983) The resolution of bimodal peaks in the analytical ultracentrifuge in the presence anf absence of ligands. Analytical Biochemistry, 131 (1). pp. 232-235.

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Abstract

The patterns for peak resolution in the analytical ultracentrifuge characteristic of systems associating by different mechanisms are analyzed. These are (i) that in which reequilibration is infinitely slow; (ii) that in which reequilihration is rapid, i.e., a Gilbert-type system; and (iii) that in which reequilibration is rapid, but the self-association is mediated by a ligand. Criteria for distinguishing the three are described and illustrated by results on tubulin self-association under various conditions.

Item Type: Article
Uncontrolled Keywords: ultracentrifuge; self-association; boundary resolution; ligand mediation of association
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 01 Jun 2011 09:45
Last Modified: 29 Oct 2018 09:41
URI: http://ir.cftri.com/id/eprint/10060

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