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Thidiazuron induced somatic embryogenesis in Coffea arabica L. and Coffea canephora P ex Fr.

Giridhar, P. and Vinod, K. and Indu, E. P. and Ravishankar, G. A. and Chandrasekar, A. (2004) Thidiazuron induced somatic embryogenesis in Coffea arabica L. and Coffea canephora P ex Fr. Acta Botanica Croatica, 63 (1). pp. 25-33. ISSN 0365–0588

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Abstract

The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s �M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions of explants on TDZ (9.08 �M) containing medium within a period of two months. Subculturing of these embryo clusters produced more secondary embryos on reduced TDZ (0.045–0.91�M) containing medium and these subsequently developed into plantlets (80–85%) on development medium followed by rooting on MS basal medium. This direct somatic embryogenesis from leaf and hypocotyl explants in Coffea sp. is a strong evidence of cell totipotency. The rapid somatic embryo induction protocol would be useful for the mass propagation, direct regeneration and genetic transformation of selected elite lines.

Item Type: Article
Uncontrolled Keywords: Cotyledons; Hypocotyls; Leaf; Somatic embryos
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 07 Life Sciences > 02 Evolution & genetics
Divisions: Plant Cell Biotechnology
Depositing User: Users 197 not found.
Date Deposited: 01 Jun 2011 09:03
Last Modified: 28 Dec 2011 10:23
URI: http://ir.cftri.com/id/eprint/10055

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