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Direct somatic embryogenesis from Coffea arabica L. and Coffea canephora P ex Fr. under the influence of ethylene action inhibitor-silver nitrate

Giridhar, P. and Indu, E. P. and Vinod, K. and Chandrashekar, A. and Ravishankar, G. A. (2004) Direct somatic embryogenesis from Coffea arabica L. and Coffea canephora P ex Fr. under the influence of ethylene action inhibitor-silver nitrate. Acta Physiologiae Plantarum, 26 (3). pp. 299-305.

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Abstract

For the first time direct somatic embryogenesis from hypocotyl explants of in vitro regenerated plantlets of C. arabica and C. canephora was achieved on modified MS medium containing 10 - 70 mM silver nitrate supplemented with 1.1 mM N6 benzyl adenine and 2.85 mM indole-3-acetic acid. A maximum of 144.1±7.3 and 68.7±3.3 embryos per explant were produced at 40 mM silver nitrate in C. canephora and C. arabica respectively. Only yellow friable embryogenic callus obtained from the cut edges of most of leaf explants of both C. arabica and C. canephora at all concentrations of silver nitrate were tried in this experiment. Formation of secondary embryos from stage I primary embryos (small yellow, round, globular embryos) was more (28.23±1.3) at 60 mM silver nitrate in C. canephora, while 40 mM silver nitrate supported more of secondary embryo formation in C. arabica (40.5±1.2). When stage II (green globular round matured embryos) and stage III primary embryos (tubular stage embryos) were used, secondary embryo formation was very small and many of these embryos developed into plantlets and some of them even rooted. By using these protocols within 45 - 60 days it is possible to get secondary embryos from primay embryos and direct somatic embryos from hypocotyls of in vitro plantlets in both these Coffea species.

Item Type: Article
Uncontrolled Keywords: Regeneration, Somatic embryos
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 07 Life Sciences > 02 Evolution & genetics
Divisions: Plant Cell Biotechnology
Depositing User: Users 197 not found.
Date Deposited: 01 Jun 2011 09:03
Last Modified: 28 Dec 2011 10:23
URI: http://ir.cftri.com/id/eprint/10053

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